Blood Sampling For Lipids
Serum total cholesterol and HDL cholesterol may be measured in a non-fasting sample. When serum triglycerides are also required the blood should be taken after a fast of 12-14h. In all cases the blood should be taken with minimal venous occlusion after the patient has been sitting for 10 minutes.
LDL is calculated from the total cholesterol, HDL cholesterol and triglyceride concentration from a fasting sample provided that the triglyceride level is below 4.5 mmol/L.