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Laboratory Medicine

Test Directory / sFlt-1/PlGF ratio test

sFlt-1/PlGF ratio test

Brown clotted serum, gel barrier

TestsFlt-1/PlGF ratio test
Common AbbreviationsPETRAT, Pre-eclampsia markers
Tube typeBrown clotted serum, gel barrier
Clinical IndicationThe sFlt-1/PlGF ratio is intended for use as an aid in the diagnosis of preeclampsia in conjunction with other diagnostic and clinical information.
Specimen TypeBlood
Sample typeSerum
Minimum Volume0.5mL
Special PrecautionsNo special requirements
StabilityStable for 48 hours at 2-8 °C, 6 months at -20 °C.
Turnaround TimeUrgent: 4 hours
Reference IntervalShould be interpreted using Obstetric PET markers SOP
LimitationsIcterus: Presence of bilirubin in concentrations up to 25 mg/dL. Haemolysis: Presence of haemoglobin in concentrations up to 500 mg/dL . Lipaemia: Presence of triglycerides in concentrations up to 1500 mg/dL . The assay is unaffected by biotin (?123 nmol/L or ?30 ng/mL). No interference was observed from rheumatoid factors up to a concentration of 600 IU/mL. Samples should not be taken from patients receiving therapy with high biotin doses (i.e. > 5 mg/day) until at least 8 hours following the last biotin administration. In vitro tests were performed on 16 commonly used pharmaceuticals. No interference with the assay was found. In rare cases, high titres of antibodies to analyte specific antibodies, streptavidin or ruthenium can occur. Effects minimised by assay design.
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