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Laboratory Medicine

Test Directory / Vitamin B12

Vitamin B12

Brown clotted serum, gel barrier

TestVitamin B12
Common AbbreviationsB12
ProfileHaematinics
Tube typeBrown clotted serum, gel barrier
Clinical IndicationMeasured to confirm the diagnosis of vitamin B12 deficiency following the identification of anaemia. Clinical and laboratory findings suggestive of B12 deficiency include neurological abnormalities, decreased serum vitamin B12 levels. macrocytic anaemia and increased excretion of methylmalonic acid.
Specimen TypeBlood
Sample typeSerum
Minimum Volume0.5mL If requesting more than 10 tests please send an additional brown clotted serum sample.
Special PrecautionsNo special requirements
Stability24 hours at 20°C, 48 hours at 2 - 8°C and 56 days at -20°C.
Turnaround TimeInpatient: 24 hours Outpatient/ GP: 24 hours
LaboratoryYork and Scarborough
Reference Interval197 - 771 ng/L (Quoted by the manufacturer)
LimitationsAnalysis should not be performed on haemolysed, icteric or lipaemic samples. Roche assay designed to avoid interference from ant-intrinsic factor antibodies. The assay is unaffected by biotin < 205 nmol/L or < 50 ng/mL. No interference was observed from rheumatoid factors up to a concentration of 1500 IU/mL. No interference was observed from IgG < 2.8 g/dL; IgA < 1.6 g/dL; IgM < 1.0 g/dL Samples should not be taken from patients receiving therapy with high biotin doses (i.e. > 5 mg/day) until at least 8 hours following the last biotin administration. In rare cases, interference due to extremely high titers of antibodies to analyte specific antibodies, streptavidin or ruthenium can occur. In vitro tests were performed on 16 commonly used pharmaceuticals. No interference with the assay was found. Presence of immunoglobulin-vitamin B12 complexes may cause high results.
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