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Laboratory Medicine

Test Directory / Vitamin D

Vitamin D

Brown clotted serum, gel barrier

TestVitamin D
Common Abbreviations25-OH VITD
Tube typeBrown clotted serum, gel barrier
Clinical IndicationVitamin D is primarily requested for the investigation of metabolic bone disease. Other indications may include hyperparathyroidism, patients with tuberculosis, individuals on anticonvulsant therapy, subjects on anti-retroviral therapy and those with CKD or nephrotic syndrome.
Specimen TypeBlood
Sample typeSerum
Minimum Volume0.5mL If requesting more than 10 tests please send an additional brown clotted serum sample.
Special PrecautionsNo special requirements
Stability72 hours at 20 - 25°C, 4 days at 2 - 8°C and 24 weeks at -20°C
Turnaround TimeInpatient: 24 hours Outpatient/ GP: 24 hours
LaboratoryYork and Scarborough
Reference Interval >50 nmol/L - replete - no treatment required 30-50 nmol/L - indicates insufficiency - treatment advised if symptomatic <30 nmol/L - indicates deficiency - treatment recommended
LimitationsAnalysis should not be performed on grossly haemolysed, icteric or lipaemic samples. The assay is unaffected by biotin < 2456 nmol/L or < 600 ng/mL. Samples should not be taken from patients receiving therapy with high biotin doses. Some studies have shown that serum concentrations of biotin can reach up to 355 ng/mL within the first hour after biotin ingestion for subjects consuming supplements of 20 mg biotin per day. In vitro tests were performed on 17 commonly used pharmaceuticals. No interference with the assay was found. In rare cases, interference due to extremely high titres of antibodies to analyte-specific antibodies, streptavidin or ruthenium can occur. These effects are minimized by suitable test design. For diagnostic purposes, the results should always be assessed in conjunction with the patientÂ’s medical history, clinical examination and other findings.
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